(1) It is generally preferable to protect RNA fragments of ∼250–400 nucleotides shorter fragments are likely to be contaminated by incompletely digested RNA fragments, while longer fragments generally give overly complex RNase T1 digestion patterns. These are designed according to the following considerations. SsDNA probes are made using recombinant phagemids, which contain an origin of replication derived from M13 (or other single‐stranded DNA phage) via standard methods. Gott, in Methods in Enzymology, 2007 3.2.1 Design and isolation of ssDNA probes
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